Journal: Frontiers in Pharmacology

Article Title: Congrong Shujing Granules ameliorates mitochondrial associated membranes to against MPP + -induced neurological damage in the cellular model of Parkinson’s disease

doi: 10.3389/fphar.2025.1509317

Figure Lengend Snippet: CRSJG improved the mitochondrial membrane potential reduction and ROS increase caused by MPP + . After treating with 1,000 μmol/L MPP + -induced SH-SY5Y cells with 2.5% CRSJG-medicated serum or 100 μmol/L 2-APB for 24 h, (A–C) Flow cytometry was used to detect ROS levels, and the quantitative results of MFI; (D,E) Flow cytometry was used to detect MMP. Results are showed as mean ± standard deviation (SD), of which all results are from four independent experiments. M: ## P < 0.01 vs. control group; * P < 0.05, ** P < 0.01 vs. MPP + group.

Article Snippet: MPP + was obtained from Macklin, China; 2-ABP was obtained from APExBIO, United States; reference substance of Rutin was obtained from Yuanye, China; reference substance of echinacoside was obtained from Alfa, China; reference substance of paeoniflorin, salvianolic acid B, acteoside and tanshinone IIa were obtained from Dilger, China; CCK-8, Fluo-3-AM fluorescent probe, Reactive oxygen species (ROS) assay kit, and JC-1 mitochondrial membrane potential (MMP) assay kit were obtained from Beyotime, China; Rhod-2-AM cell permeable calcium ion fluorescence probe Yeasen, China; IP 3 R, VDAC, GRP75, Cyt-C, Bax, Bcl-2, Caspase-3, and Caspase-9 antibodies were obtained from Proteintech, China; Cell apoptosis kit and MCU antibodies were obtained from Abbkine, China; MCU antibodies and BCA Protein Assay Kit were obtained from Boster, China; DMEM medium was obtained from Gibco, United States; Origin fetal bovine serum (FBS) was obtained from MRC, United States.

Techniques: Membrane, Flow Cytometry, Standard Deviation, Control

Journal: Redox Report : Communications in Free Radical Research

Article Title: Varespladib attenuates Naja atra -induced acute liver injury via reversing Nrf2 signaling-mediated ferroptosis and mitochondrial dysfunction

doi: 10.1080/13510002.2025.2507557

Figure Lengend Snippet: Varespladib relieves N. atra -induced mitochondrial dysfunction and suppresses N. atra -induced excessive mitophagy via Nrf2 signaling. (A) ATP production in L02 cells. (B) CS activity in L02 cells. (C) Mitochondrial ROS in L02 cells. (D) MMP in L02 cells. (E) Protein levels of PINK1, Parkin, and LC3-II/I were measured by Western blot analysis in L02 cells. (F–H) and the quantitative maps were created. * p < 0.05, ** p < 0.01, and *** p < 0.001. ns, not significant.

Article Snippet: C0533), CCK-8 Kit, Cell Mitochondria Isolation kit, Assay kits for ATP production, mitochondrial membrane potential (MMP), ROS, and apoptosis were obtained from Beyotime Co., Ltd. (Shanghai, China).

Techniques: Activity Assay, Western Blot